In Vitro Transcribed (IVT) mRNA provides a flexible and cost-effective platform to produce mRNA transcripts that can be used in many applications including therapeutics and vaccine development, gene editing and gene expression studies, antibody development, and more. IVT mRNAs can be transcribed from circular plasmid DNA, PCR products, and double-stranded oligos that contain the promoter sequences of phage RNA polymerases, most commonly T7.

Eurofins Genomics Blue Heron offers various services from DNA template options to IVT mRNA production. All our mRNA products are capped and polyadenylated to ensure stability and downstream application performance. Our standard QC and characterizations include size, purity, and integrity. Additional QC methods such as capping efficiency are available.

 

Quality Control and Specification of mRNA

QC Item Test Method Specification Standard Upgrade
Appearance Visual inspect Clear, no visible particles Yes Yes
pH pH meter Target ± 0.5 Yes Yes
Concentration Nanodrop 0.5-1mg/ml Yes Yes
Purity Nanodrop 260/280 is 1.8-2.3, 230/280 ratio is 2.0-2.3 Yes Yes
RNA Length and Integrity Gel electrophoresis Expected fragment size observed Yes Yes
RNA Length and Integrity Capillary electrophoresis DV200 metric evaluation No Yes
Capping Efficiency Enzyme digestion and gel electrophoresis ≥ 90% No Yes
Endotoxin Kinetic turbidimetric assay (KTA) – limulus amebocyte lysate (LAL) < 10EU/mg No Yes

How to Order

Order Online

Login to the ordering site and click the IVT mRNA links under Order Now.

Manual Request

Fill out the order form (right) and email it to sales@blueheronbio.com.